Estimating the persistence length of a worm-like chain molecule from force-extension measurements.
نویسندگان
چکیده
We describe a simple computation of the worm-like chain model and obtain the corresponding force-versus-extension curve. We propose an improvement to the Marko and Siggia interpolation formula of Bustamante et al (Science 1994, 265:1599-1600) that is useful for fitting experimental data. We apply it to the experimental elasticity curve of single DNA molecules. Finally, we present a tool to study the agreement between the worm-like chain model and experiments.
منابع مشابه
DNA entropic elasticity for short molecules attached to beads
Single-molecule experiments in which force is applied to DNA or RNAmolecules have enabled important discoveries of nucleic acid properties and nucleic acid-enzyme interactions. These experiments rely on a model of the polymer force-extension behavior to calibrate the experiments; typically the experiments use the worm-like chain (WLC) theory for double-stranded DNA and RNA. This theory agrees w...
متن کاملStudies of translation , on the ensemble level using in vivo techniques and on the single molecule level using optical
With the evolution of single molecule techniques as force-scope optical tweezers, it has become possible to perform very accurate measurements of the elastic properties of biopolymers as e.g. DNA. Nucleic acid elasticity is important in the interaction of these molecules with proteins and protein complexes in the living cell. Mostexperimental and theoretical effort has been aimed at uncover...
متن کاملMolecular mechanics of cardiac titin's PEVK and N2B spring elements.
Titin is a giant elastic protein that is responsible for the majority of passive force generated by the myocardium. Titin's force is derived from its extensible I-band region, which, in the cardiac isoform, comprises three main extensible elements: tandem Ig segments, the PEVK domain, and the N2B unique sequence (N2B-Us). Using atomic force microscopy, we characterized the single molecule force...
متن کاملExplicit and implicit learning and maps of cortical motor output.
Squares are experimental force versus extension data for 97 kb X-DNA dimers from figure 3 of (2); solid line is a fit of the entropic force required to extend a worm-like polymer. The fit parameters are the DNA length (L = 32.80 + 0.10 plm) and the persistence length (A = 53.4 ± 2.3 nm). Shown for comparison (dashed curve) is the freely jointed chain model (2) with L = 32.7 KILm and a segment l...
متن کاملPulling-speed-dependent force-extension profiles for semiflexible chains.
We present theory and simulations to describe nonequilibrium stretching of semiflexible chains that serve as models of DNA molecules. Using a self-consistent dynamical variational approach, we calculate the force-extension curves for worm-like chains as a function of the pulling speed, v(0). Due to nonequilibrium effects the stretching force, which increases with v(0), shows nonmonotonic variat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Biophysical journal
دوره 76 1 Pt 1 شماره
صفحات -
تاریخ انتشار 1999